1. 个人简介：

王文恭，教授，博士生导师，任职于北京大学基础医学院生物化学与分子生物学系。

2. 研究方向与领域：

主要研究方向：血管衰老及相关心血管疾病的转录后调控，特别集中于RNA结合蛋白，非编码RNA及RNA甲基化修饰的作用。

基金项目：

（1）国家自然科学基金重点项目“HuR调控的衰老相关代谢紊乱对血管炎症与动脉粥样硬化的影响机制”，81930035，297万，2020.1-2024.12.

（2）国家自然科学基金重大研究计划重点项目“血管衰老过程中NSUN2与METTL3/ METTL14对衰老相关基因mRNA的协同甲基化以及血清m6A与m5C水平分析”, 200万，2018.1-2021.12

（3）国家重点研发计划“胞内及微环境RNA-蛋白质复合机器对细胞命运的调控作用及机制”的子课题“RNA-蛋白质复合机器在转录及转录后水平的调控机制研究”，课题组长，2017YFA0504302，634万，2017.7-2022.6

3. 实验室成员：

易霞：副教授Email: yixia@hsc.pku.edu.cn

姜彬：副主任技师Email: jiangbin@bjmu.edu.cn

在读博士研究生：李秀、刘词航、吴崇阳、吴东兴、苏新萍、盛杰、于泽农、申凌翰

在读硕士研究生：林韵萍

      4. 主要代表作：

（1）Zhang Z, Zong C, Jiang M, Hu H, Cheng X, Ni J, Yi X, Jiang B, Tian F, Chang MW, Su W, Zhu L,Li J, Xiang X, Miao C, Gorospe M, de Cabo R, Dou Y, Ju Z, Yang J, Jiang C, Yang Z, Wang W. Hepatic HuR Modulates Lipid Homeostasis in Response to High-Fat Diet. Nature Commun. 2020 Jun 16;11(1):3067.

（2）Hu H, Jiang M, Cao Y, Zhang Z, Jiang B, Tian F, Feng J, Dou Y, Gorospe M, Zheng M, Zheng L, Yang Z, Wang W.HuR Regulates Phospholamban Expression in Isoproterenol-Induced Cardiac Remodelling. Cardiovasc Res. 2020 Apr 1;116(5):944-955.

（3）Tang H, Wang H, Cheng X, Fan X, Yang F, Zhang M, Chen Y, Tian Y, Liu C, Shao D, Jiang B, Dou Y, Cong Y, Xing J, Zhang X, Yi X, Songyang Z, Ma W, Zhao Y, Wang X, Ma J, Gorospe M, Ju Z, Wang W. HuR Regulates Telomerase Activity through TERC Methylation. Nature Commun. 2018 Jul 10;9(1):2721.

（4）Li Q, Li X, Tang H, Jiang B, Dou Y, Gorospe M, Wang W.NSUN2-Mediated m5C Methylation and METTL3/METTL14-Mediated m6AMethylation Cooperatively Enhance p21 Translation. J Cell Biochem.2017 Sep;118 (9):2587-2598.

（5）Luo Y, Feng J, Xu Q, Wang W, Wang X.NSUN2Deficiency Protects Endothelium From Inflammation via mRNA Methylation of ICAM- 15. Circulation Res. 2016 Mar 18;118(6):944-56.

（6）Wang W.mRNAMethylation by NSUN2 in Cell Proliferation.Wiley Interdiscip Rev RNA. 2016 Nov;7(6):838-842.

（7）Cai X, Hu Y, Tang H, Hu H, Pang L, Xing J, Liu Z, Luo Y, Jiang B, Liu T, Gorospe M, Chen C, Wang W. RNA Methyltransferase NSUN2 Promotes Stress-induced HUVEC Senescence. Oncotarget. 2016 Apr 12;7(15):19099-110.

（8）Tang H, Fan X, Xing J, Liu Z, Jiang B, Dou Y, Gorospe M, Wang W.NSun2Delays Replicative Senescence by Repressingp27(KIP1) Translation andElevating CDK1 Translation. Aging (Albany NY). 2015 Dec;7(12):1143-58.

（9）Xing J, Yi J, Cai X, Tang H, Liu Z, Zhang X, Martindale JL, Yang X, Jiang B, Gorospe M, Wang W.NSun2 Promotes Cell Growth via Elevating CDK1 Translation. Mol Cell Biol. 2015 Dec;35(23):4043-52

（10）Nie J, Jiang M, Zhang X, Tang H, Jin H, Huang X, Yuan B, Zhang C, Lai JC, Nagamine Y, Pan D, Wang W, Yang Z. Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.Cell Rep. 2015 Oct 27;13(4):723-732.Co-correspondence

（11）Wang W. HuR and Post-transcriptional Regulation in Vascular Aging.Sci China Life Sci. 2014 Aug;57(8):863-6.

（12）Yuan S, Tang H, Xing J, Fan X, Cai X, Li Q, Han P, Luo Y, Zhang Z, Jiang B, Dou Y, Gorospe M, Wang W.Methylation byNSun2Represses the Levels and Function of miR-125b. Mol Cell Biol.2014 Oct 1;34(19):3630-41.

（13）Xu F, Pang L, Cai X, Liu X, Yuan S, Fan X, Jiang B, Zhang X, Dou Y, Gorospe M, Wang W.let-7-repressesed Shc Translation Delays Replicative Senescence. Aging Cell. 2014Feb;13(1):185-92.

（14）Pang L, Tian H, Chang N, Yi J, Xue L, Jiang B, Gorospe M, Zhang X, Wang W.Loss of CARM1 is Linked to Reduced HuR Function in Replicative Senescence.BMC Mol Biol.2013 Jul 9;14:15.

（15）Zhang X, Liu Z, Yi J, Tang H, Xing J, Yu M, Tong T, Shang Y, Gorospe M, Wang W. NSun2 Stabilizes p16INK4 mRNA by Methylating the p16 3’UTR.NatureCommun.2012 Mar 6;3:712.

（16）Wang W.Regulatory RNA-binding Proteins in Senescence.Ageing Res Rev. 2012 Sep;11(4):485-90.

（17）Xu F, Zhang X, Lei Y, Liu X, Liu Z, Tong T, Wang W.Loss of Repression of HuR Translation by miR-16 may be Responsible for the Elevation of HuR in Human Breast Carcinoma.J Cell Biochem. 2010 Oct 15;111(3):727-34.

（18）Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, and Wang W.HuR Uses AUF1 as a Cofactor to Promote p16INK4 mRNA Decay.Mol Cell Biol.2010 Aug;30(15):3875-86.

（19）Guo G, Ma L, Jiang B, Yi J, Tong T, and Wang W. Hydrogen Peroxide Induces p16INK4through an AUF1-dependent Manner.J Cell Biochem.2010 Apr 1;109(5):1000-5.

（20）Yi J, Chang N, Liu X, Guo G, Xue L, Tong T, Gorospe M, Wang W. Reduced nuclear export of HuR mRNA by HuR is linked to the loss of HuR in replicative senescence.Nucleic Acids Res. 2010 Mar;38(5):1547-58.

（21）Ma L, Chang N, Guo S, Zhang Z, Wang W, and Tong T. CSIG Regulates PTEN Translation in Replicative Senescence. Mol. Cell. Biol. 2008,Oct;28, 6290-301.Co-correspondence

（22）Wu J, Xue L, Weng M, Sun Y, ZhangZ, Wang W, and Tong T.Sp1 is Essential for p16INK4a Expression in Human Diploid Fibroblasts during Senescence. PlosOne,2007 Jan 17;2(1):e164. Co-cprrespondence

（23）Wang W, Yang X, Martindale J, and Gorospe M. AUF1-Mediated mRNA Turnover is Responsible for Up-regulation of p16 during Replicative Senescence. EMBO Rep. 2005 Feb;6(2):158-64.

（24）Wang W, Yang X, Kawai T, Lopez De Silanes I, Mazan-Mamczarz K, Chen P, Chook Y, Quensel C, Kohler M, Gorospe M. AMPK-Regulated Acetylation and Phosphorylation of Importin-a1: Involvement in the Nuclear Import of RNA-binding Protein HuR. J Biol Chem. 2004 Nov 12;279(46):48376-88. Co-correspondence

（25）Wang W, Yang X, Lopez I, Carking D, and Gorospe M. 2003. Increased AMP: ATP Ratio and AMP-activated Protein Kinase Activity during Cellular Senescence Linked to Reduced HuR Function.J Biol Chem. 2003 Jul 18;278(29):27016-23.

（26）Wang W, Fan J, Yang X, Furer-Galban S, Lopez I, Kobbe C, Guo J, Georas S, Foufelle F, Hardie D. Carking D, and Gorospe M. AMP-Activated Kinase Regulates Cytoplasm HuR, Mol Cell Biol. 2002 May;22(10):3425-36.

（27）Wang W, Yang X, Cristofalo V, Holbrook N, and Gorospe M. Loss of HuR is Linked to Reduced Expression of Proliferative Genes during Replicative Senescence.Mol Cell Biol. 2001 Sep;21(17):5889-98.

（28）Wang W, Caldwell MC, Lin S, Furneaux H, and Gorospe M. HuR Regulates Cyclin A and Cyclin B1 mRNA Stability during Cell Proliferation. EMBO J. 2000 May 15;19(10):2340-50.

（29）Wang W, Furneaux H, Caldwell MC, Hutter C, Holbrook N, and Gorospe M. HuR Regulates p21 mRNA Stabilization by Ultraviolet light. Mol Cell Biol. 2000 Feb;20(3):760-9.

（30）Wang W and Passaniti A. Extracellular Matrix Inhibits Apoptosis and Enhances Endothelial Differentiation by a NF-kB-dependent Mechanism. J Cell Biochem. 1999 Jun 1;73(3):321-31.

5．联系方式:

王文恭

北京大学基础医学院生物化学与分子生物学系

Email: wwg@bjmu.edu.cn

电话：86-10-82802949